Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal

RRID Number: AB_2860483
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal
经验证的应用
ELISA(Cap),FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse IL2RG
免疫原
Recombinant Mouse CD132 / IL2RG Protein (Catalog#50087-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD132 / IL2RG (rM CD132 / IL2RG; Catalog#50087-M08H; NP_038591.1; Met1-Ala263).
来源
Monoclonal Rabbit IgG Clone #011
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Mouse CD132 / IL2RG in ELISA pair set (Catalog: # SEK50087). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 50087-R005).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of mouse IL2RG in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IL2RG monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Flow cytometric analysis of Mouse IL2RG(CD132) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IL2RG(CD132), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal: 别称

Anti-CD132 Antibody; Anti-gamma(c) Antibody; Anti-gc Antibody; Anti-[g]c Antibody

IL2RG 背景信息

The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor subunit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15, and the interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (X-SCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor based on chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why a deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.
全称
interleukin 2 receptor, gamma
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Russell SM, et al. (1993) Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor. Science. 262 (5141): 1880-3.
  • Miyazaki T, et al. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science. 266 (5187): 1045-7.
  • Takeshita T, et al. (1992) Cloning of the gamma chain of the human IL-2 receptor. Science. 257 (5068): 379-82.
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