Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal

RRID Number: AB_2860499
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal
经验证的应用
ELISA,FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse LDLR/LDL Receptor
No cross-reactivity in ELISA with
Human LDLR / LDL Receptor
免疫原
Recombinant Mouse LDLR protein (Catalog#50305-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR / LDL Receptor (rM LDLR; Catalog#50305-M08H; Met 1-Arg 790; NP_034830.2).
来源
Monoclonal Rabbit IgG Clone #032
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:5000-1:10000
ICC-IF 1:50-1:1000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-LDLR/LDL Receptor Antibody, Rabbit Monoclonal: 别称

Anti-Hlb301 Antibody

LDLR/LDL Receptor 背景信息

LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
全称
low density lipoprotein receptor
参考文献
  • Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
  • Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
  • Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
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