Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal

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Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Mouse
特异性
Mouse methionyl aminopeptidase 2/METAP2
免疫原
Recombinant Mouse MAP2 / METAP2 protein (Catalog#50528-M08B)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse MAP2 / METAP2 (rM MAP2 / METAP2; Catalog#50528-M08B; O08663; Ala2-Tyr478).
来源
Monoclonal Rabbit IgG Clone #010
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal 图片

Anti-METAP2 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:65 kDa

Mouse METAP2 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-Mouse METAP2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse METAP2 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 52 kDa

Observed band size: 72 kDa

Recombinant Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Monoclonal: 别称

Anti-4930584B20Rik Antibody; Anti-A930035J23Rik Antibody; Anti-AI047573 Antibody; Anti-AL024412 Antibody; Anti-Amp2 Antibody; Anti-AU014659 Antibody; Anti-Mnpep Antibody; Anti-p67 Antibody; Anti-p67eIF2 Antibody

methionyl aminopeptidase 2/METAP2 背景信息

METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
全称
methionyl aminopeptidase 2
参考文献
  • Bennett, et al. (1997) EPR Studies on the Mono- and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.
  • Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.
  • Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
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