Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Mouse
特异性
Mouse Semaphorin 4D
免疫原
Recombinant Mouse Semaphorin 4D / SEMA4D / CD100 protein (Catalog#50642-M08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Semaphorin 4D / SEMA4D / CD100 (rM Semaphorin 4D / SEMA4D / CD100; Catalog#50642-M08H; NP_038688.2; Met1-Arg733).
来源
Monoclonal Rabbit IgG Clone #010
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Mouse SEMA4D in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse SEMA4D monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-Semaphorin 4D Antibody, Rabbit Monoclonal: 别称

Anti-CD100 Antibody; Anti-coll-4 Antibody; Anti-Semacl2 Antibody; Anti-Semaj Antibody; Anti-Semcl2 Antibody

Semaphorin 4D 背景信息

Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
全称
semaphorin 4D
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