Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Mouse
特异性
Mouse UCHL1
Has cross-reactivity in ELISA with
Human UCHL1
Human UCHL3
Rat UCHL1
免疫原
Recombinant Mouse UCHL1 protein (Catalog#50690-M07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCHL1 (rM UCHL1; Catalog#50690-M07E; Q9R0P9; Gln2-Ala223).
来源
Monoclonal Rabbit IgG Clone #011
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal 图片

Anti-UCHL1 rabbit monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:27 kDa

Mouse UCHL1 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg U87MG Whole Cell Lysate

2 µL anti-Mouse UCHL1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse UCHL1 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Recombinant Anti-UCHL1 Antibody, Rabbit Monoclonal: 别称

Anti-AW822034 Antibody; Anti-C88048 Antibody; Anti-gad Antibody; Anti-PGP9.5 Antibody; Anti-R75593 Antibody; Anti-UCH-L1 Antibody; Anti-UCHL-1 Antibody

UCHL1 背景信息

Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.
全称
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
参考文献
  • Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92.
  • Choi J., et al., 2004, J. Biol. Chem. 279:13256-64.
  • Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41
  • Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44.
  • Zetterberg, M. et al., 2010, Mol Neurodegener  5 :11.
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