Anti-Myc tag Antibody, Mouse Monoclonal

RRID Number: AB_2860041

Anti-Myc tag Antibody, Mouse Monoclonal 产品信息

Anti-Myc tag Antibody, Mouse Monoclonal
Myc tag
A synthetic Myc tag peptide conjugated to KLH
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #07
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
IP 1-4 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody, Mouse Monoclonal 图片

MYC tag was immunoprecipitated using:

Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MYC tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Myc tag 背景信息

The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Hillman MC, et al. (2001) A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif 23 (2): 359-368.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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