Anti-Myc tag Antibody, Mouse Monoclonal

RRID Number: AB_2860041
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Anti-Myc tag Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-Myc tag Antibody, Mouse Monoclonal
经验证的应用
IP
特异性
Myc tag
免疫原
A synthetic Myc tag peptide conjugated to KLH
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #07
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
IP 1-4 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody, Mouse Monoclonal 图片

MYC tag was immunoprecipitated using:

Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MYC tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Myc tag 背景信息

The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
参考文献
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Hillman MC, et al. (2001) A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif 23 (2): 359-368.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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