应用 | 推荐稀释比/用量 |
---|---|
IP | 1-4 μg/mg of lysate |
MYC tag was immunoprecipitated using:
Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate
Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate
2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
Primary antibody:
Anti-MYC tag mouse monoclonal antibody,at 1:100 dilution
Secondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa