Anti-ADRB3 Antibody, Rabbit Polyclonal

1/2
Price:
Size:
Number:

Anti-ADRB3 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ADRB3 Antibody, Rabbit Polyclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Rat
特异性
Rat ADRB3
免疫原
A synthetic peptide corresponding to the C-terminus of the Rat ADRB3
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Rat ADRB3, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ADRB3 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ADRB3 Antibody, Rabbit Polyclonal 图片

Anti-Adrb3 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:35 kDa

Adrb3 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

1 µL anti-Adrb3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Adrb3 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size: 35 kDa

ADRB3 背景信息

beta3-Adrenergic receptor (ADRB3) Trp64Arg polymorphism is associated with atherogenic risk factors that include weight gain, insulin resistance, and diabetes. Habitual exercise brings higher cardiorespiratory fitness and results in the amelioration of atherosclerotic risk factors. Presence of the beta3-Adrenergic receptor (ADRB3) gene Trp64Arg (T64A) polymorphism may be associated with an increased susceptibility for essential hypertension (EH). The beta3-adrenergic receptor (ADRB3) regulates thermogenesis and lipolysis in brown and white adipose tissue. ADRB3 plays a critical role in the regulation of energy metabolism in mammals. Polymorphism in the ovine ADRB3 gene has been reported to be associated with birth weight, growth rate, carcass composition, and cold survival.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.