Anti-Galectin-1 Antibody, Rabbit Polyclonal

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Anti-Galectin-1 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Galectin-1 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Rat
特异性
Rat Galectin-1
免疫原
Recombinant Rat Galectin-1 / LGALS1 Protein (Catalog#80248-RNAE)
制备方法
Produced in rabbits immunized with purified, recombinant Rat Galectin-1 / LGALS1 (rR Galectin-1 / LGALS1; Catalog#80248-RNAE; P11762; Ala2-Glu135). Galectin-1 / LGALS1 specific IgG was purified by Rat Galectin-1 / LGALS1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin-1 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin-1 Antibody, Rabbit Polyclonal 图片

rat Galectin1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-rat Galectin1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-rat Galectin1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Observed band size: 15 kDa

Anti-Galectin1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:14 kDa

Galectin-1 背景信息

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.
全称
lectin, galactoside-binding, soluble, 1
参考文献
  • Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.
  • Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.
  • Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.
  • Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.
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