Anti-HGF Antibody, Rabbit Polyclonal

1/3
Price:
Size:
Number:

Anti-HGF Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-HGF Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IHC-P
交叉反应
Reacts with: Rat
特异性
Rat HGF
免疫原
Recombinant Rat HGF/Hepatocyte Growth Factor Protein (Catalog#80429-RNAH)
制备方法
Produced in rabbits immunized with purified, recombinant Rat HGF/Hepatocyte Growth Factor (rR HGF/Hepatocyte Growth Factor; Catalog#80429-RNAH; NP_058713.1; Met1-Leu728). HGF/Hepatocyte Growth Factor specific IgG was purified by Rat HGF/Hepatocyte Growth Factor affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HGF Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:200-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HGF Antibody, Rabbit Polyclonal 图片

Anti-HGF rabbit polyclonal antibody at 1:500 dilution

Lane A: Rat liver tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:83 kDa

Observed band size:93 kDa

Immunochemical staining of rat HGF in rat liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of rat HGF in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

HGF 背景信息

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
全称
hepatocyte growth factor (hepapoietin A; scatter factor)
研究领域
参考文献
  • Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
  • Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
  • Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
  • Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.