Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody

RRID Number: AB_2860278
EliteRmab®, manufactured by recombinant technology
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Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody 产品信息

产品名称
Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody
经验证的应用
ELISA(Cap),Microneutralizaiton(MN)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
RSV RSV Fusion
Has cross-reactivity in ELISA with
recomibnantF protein of A type of long strain (40039-V08B)
A2 strain (11049-V08B)
RSS2 strain (40037-V08B)
B type of B1 strain
免疫原
Recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F protein (Catalog#11049-V08B)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F (Catalog#11049-V08B; P03420.1; Met1-Thr529) and was produced using recombinant antibody technology.
来源
Monoclonal Rabbit IgG Clone #R338
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
内毒素
Please contact us for more information.
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody 经验证的应用

Notes
ELISA(Cap): This antibody will detect Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein / RSV-F in ELISA pair set (Catalog: # SEK11049). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 11049-R302).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody 图片

The neutralization activity of RSV F protein antibody is Measured by Microneutralization test in vitro. The cytopathic effect of VERO cells induced by 100 pfu of RSV A-A2 strain is neutralized by increasing concentrations of anti RSV Monoclonal Antibody (Catalog # 11049-R338). The neutralizing titer (IC50) of antibody is 0.6-2.3 μg/mL.

Data is provided by the customer.

Sino Biological. InC has not validated this application.

Human respiratory syncytial virus (RSV) Fusion glycoprotein / RSV-F Neutralizing Antibody: 别称

Anti-F Antibody; Anti-HRSVgp08 Antibody

RSV Fusion 背景信息

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
参考文献
  • Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8.
  • Jose A M. et al., 1997, J Gen Virol. 78: 2411-8.
  • Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7.
  • Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83.
  • Trento A. et al., 2006, J Virol. 80 (2): 975-84.
  • Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8.
  • Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.
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