Antibody VH & VL Gene Cloning Service Description

Your Most Reliable & Cost-Effective Gene & Vector Supporting Partner

     

Gene Cloning Gene Synthesis

 

Expression Vector Construction

 

Plasmid Production

 

qPCR Primer Design & Testing

Mab Antibody VL and VH Gene Cloning Service Description

In many cases, one often need to clone an antibody's Light and Heavy chain genes from a mouse or rabbit hybridoma cell line. For example, one may need to rescue a contaminated hybridoma cell line or an unstable hybridoma cell line for large scale antibody production. By cloning out the antibody's light and heavy chain genes, one could produce large quantities of the antibody by transient transfection technology or by stable CHO cell line. In development of therapeutic antibody candidate using the classical hybridoma technology, once a suitable antibody is obtained, one would need to humanize the antibody and develop a high-yield stable cell line for commercialization, and hence cloning the antibody's H and L chain genes out from the original hybridoma cell line becomes a must-do task.

Cloning out a monoclonal antibody VH and VL genes from hybridoma cells can be straight forward but sometimes can be challenging as well. In many cases, the hybridoma cells may not be a true clone and hence one could clone out multiple VH and VL genes. In some cases, the hybridoma cell may contain multiple pseudo genes. Experience and expertise is critical for success.

Sino Biological has successfully cloned out hundreds of monoclonal antibody genes from hybridoma cell cultures. After cloning out the antibody genes, customers can further take advantage of our humanization expertise and advanced antibody production platform to accelerate product development.

How to Request for Antibody Gene Cloning Service?

If you are interested in using our antibody gene cloning service, please contact us by leaving an on-line message, filling out an on-line inquiry form, or sending us your detailed requests to CRO-service@sinobiological.com. A formal quote will be send back to you for your review, comment, and/or acceptance.

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Gene Cloning CRO Service+
- Gene Cloning Service Description
- Antibody VL & VH Gene Cloning Service
Antibody VH & VL Gene Cloning Service Description
- Human Gene Cloning Service
- Mouse Gene Cloning Service
- Rat Gene Cloning Service
- Cynomolgus Gene Cloning Service
- Rhesus Gene Cloning Service
- Ferret Gene Cloning Service