Antibody VL & VH Gene Cloning Service

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Antibody VL & VH Gene Cloning Service

Antibody VH & VL Gene Cloning Service Description

In many cases, one often need to clone an antibody's Light and Heavy chain genes from a mouse or rabbit hybridoma cell line. For example, one may need to rescue a contaminated hybridoma cell line or an unstable hybridoma cell line for large scale antibody production. By cloning out the antibody's light and heavy chain genes, one could produce large quantities of the antibody by transient transfection technology or by stable CHO cell line. In development of therapeutic antibody candidate using the classical hybridoma technology, once a suitable antibody is obtained, one would need to humanize the antibody and develop a high-yield stable cell line for commercialization, and hence cloning the antibody's H and L chain genes out from the original hybridoma cell line becomes a must-do task.

Cloning out a monoclonal antibody VH and VL genes from hybridoma cells can be straight forward but sometimes can be challenging as well. In many cases, the hybridoma cells may not be a true clone and hence one could clone out multiple VH and VL genes. In some cases, the hybridoma cell may ......... <Learn More>

Other Gene Cloning Services at Sino Biological

Biology CRO Service
Biology CRO Service Introduction
Antibody Production & Development CRO Service
Protein Production CRO Service
Transient Transfection Service: Mammalian Cell Culture
Molecular Biology CRO Service
Molecular Biology Service Description
Gene Cloning CRO Service+
- Gene Cloning Service Description
- Antibody VL & VH Gene Cloning Service
Antibody VH & VL Gene Cloning Service Description
- Human Gene Cloning Service
Human Gene Cloning Service Description
- Mouse Gene Cloning Service
Mouse Gene Cloning Service Description
- Rat Gene Cloning Service
Rat Gene Cloning Service Description
- Cynomolgus Gene Cloning Service
Cynomolgus Gene Cloning Service Description
- Rhesus Gene Cloning Service
Rhesus Gene Cloning Service Description
- Ferret Gene Cloning Service
Ferret Gene Cloning Service Description
Gene Expression Vector Construction CRO Service
Gene Synthesis CRO Service
Real-time PCR Service: Gene Expression Analysis by qPCR