FGF2 Protein, Human, Recombinant

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FGF2 Protein, Human, Recombinant: 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg protein as determined by the LAL method
生物活性
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.02-0.1 ng/ml.
蛋白构建
A DNA sequence encoding the mature form of human bFGF (NP_001997.5) (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.
NP No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant human bFGF consists of 147 amino acids and predicts a molecular mass of 16.5 kDa.
缓冲液
Lyophilized from sterile 20mM Tris, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF2 Protein, Human, Recombinant: 图片

FGF2 Protein, Human, Recombinant: 别称

BFGF Protein, Human; FGF-2 Protein, Human; FGFB Protein, Human; HBGF-2 Protein, Human

FGF2 背景信息

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

bFGF/FGF2 Protein & Antibody Products

全称
fibroblast growth factor 2 (basic)
参考文献
  • Takayama S,et al. (2001) Periodontal regeneration by FGF-2 (bFGF) in primate models. J Dent Res. 80(12): 2075-9.
  • Niu YJ, et al. (2004) Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chin Med J (Engl). 117(2): 252-7.
  • Zhang Y,et al. (2004) Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chin Med J (Engl). 117(4): 601-3.
  • Establishment of a Rabbit Oct4 Promoter-Based EGFP Reporter System
    Author
    Quan, L;Chen, Y;Song, J;Yan, Q;Zhang, Q;Lai, S;Fan, N;Xin, J;Zou, Q;Lai, L;
    Year
    2014
    Journal
    PLoS ONE
    Application
    culture
  • A lab-centred approach for initiating early stage regeneration of dental pulp using specialised alginate biomimetic microenvironments
    Author
    Bhoj, M;
    Year
    2015
    Journal
    HKU Theses Online (HKUTO)
  • Expression of functional recombinant human fibroblast growth factor 8b and its protective effects on MPP(+)-lesioned PC12 cells
    Author
    Chen, N;Ma, J;Zhao, Y;Wu, M;Yang, H;Gong, W;Chao, J;Li, X;
    Year
    2015
    Journal
    Appl. Microbiol. Biotechnol.
    Application
    cell-based
  • Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion
    Author
    Yang, X;Steinberg, F;Zhuang, L;Bessey, R;Trueb, B;
    Year
    2016
    Journal
    Int. J. Mol. Med.
    Application
    cell-based
  • PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs
    Author
    Shan, Y;Liang, Z;Xing, Q;Zhang, T;Wang, B;Tian, S;Huang, W;Zhang, Y;Yao, J;Zhu, Y;Huang, K;Liu, Y;Wang, X;Chen, Q;Zhang, J;Shang, B;Li, S;Shi, X;Liao, B;Zhang, C;Lai, K;Zhong, X;Shu, X;Wang, J;Yao, H;Chen, J;Pei, D;Pan, G;
    Year
    2017
    Journal
    Nat Commun
  • Androgen Promotes Differentiation of PLZF+ Spermatogonia pool via Indirect Regulatory Pattern
    Author
    Wang, J;Li, J;Gu, Y;Xia, Q;Song, W;Zhang, X;Yang, Y;Wang, W;Li, H;Zou, K;
    Year
    2018
    Journal
    bioRxiv
    Application
    cell-based
  • In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells
    Author
    Zhao, Y;Ye, S;Liang, D;Wang, P;Fu, J;Ma, Q;Kong, R;Shi, L;Gong, X;Chen, W;Ding, W;Yang, W;Zhu, Z;Chen, H;Sun, X;Zhu, J;Li, Z;Wang, Y;
    Year
    2018
    Journal
    Stem Cell Reports
    Application
    cell-based (hESC/iPS)
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