Anti-beta-Actin Antibody

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Anti-beta-Actin Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-beta-Actin Antibody
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human beta-Actin
免疫原
A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-beta-Actin Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IP 1-4 uL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-beta-Actin Antibody (Rabbit Polyclonal 抗体) 图片

ACTB was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-ACTB rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ACTB rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Anti-ACTB,beta-actin cytoplasmic1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: Mouse brain tissue lysate Whole Cell Lysate

Lane D: Mouse brain tissue lysate

Lane E: Rabbit brain tissue lysate

Lane F: Rabbite slpeen tissue lysate

Lane G: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:42 kDa

Anti-beta-Actin Antibody: 别称

Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody

beta-Actin 背景信息

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.
全称
actin, beta
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