Antibody Purification and Immunoprecipitation (IP)

Immunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other techniques such as gel filtration or density gradient sedimentation. The source of antigen for immunoprecipitation can be unlabeled cells or tissues, metabolically or intrinsically labeled cells, or in vitro-translated proteins. This unit describes a wide range of immunoprecipitation techniques, using either suspension or adherent cells lysed by various means.Usually, plasmas transfected cells or natural cells are ideal sample for immunoprecipitation.

Follow the immunoprecipitation procedure if protein A/G/L were conjugated to magnetic beads or resins. Using the characteristic of proteinA/G/L bing to antibody's Fc region. We can purify antibody from serum or cell culture medium.