ASAH2 Proteins, Antibodies, cDNA Clones Research Reagents

All ASAH2 reagents are produced in house and quality controlled, including 4 ASAH2 Antibody, 14 ASAH2 Gene, 1 ASAH2 Lysate, 1 ASAH2 Protein, 1 ASAH2 qPCR. All ASAH2 reagents are ready to use.

ASAH2 Protein (1)

    ASAH2 Antibody (4)

      ASAH2 cDNA Clone (14)

      NM_001143974.1

      In expression vector

      NM_018830.1

      克隆载体 cDNA 产品

      In lentiviral vector

      ASAH2 qPCR Primer (1)

      ASAH2 Lysate (1)

        ASAH2 分子背景

        ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

        ASAH2 参考文献

        • Tani M, et al. (2000) Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem. 275(15): 11229-34.
        • Franzen R, et al. (2002) Nitric oxide induces neutral ceramidase degradation by the ubiquitin/proteasome complex in renal mesangial cell cultures. FEBS Lett. 532(3): 441-4.
        • Kono M, et al. (2006) Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids. J Biol Chem. 281(11): 7324-31.

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