The two methods have the same characteristics, two of which are based on specific reaction of antibodies and antigens. At the same time, these two methods have their advantages and disadvantages. It is not easy to determine which method is more sensitive or best method, because the different target molecule expression of the situation is different, the use of different methods to detect when the results will not be entirely consistent. What is the main purpose of the researchers? If the purpose is more want to know the expression of the target molecule in tissue, the immunohistochemsitry (IHC) is can be chosen. IF the purpose is to confirm the detailed expression location in tissue or the the expression of the situation or the location of the target in cells, immunofluorescence (IF) can be chosen.