Immunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other techniques such as gel filtration or density gradient sedimentation. The source of antigen for immunoprecipitation can be unlabeled cells or tissues, metabolically or intrinsically labeled cells, or in vitro-translated proteins. This unit describes a wide range of immunoprecipitation techniques, using either suspension or adherent cells lysed by various means.Usually, plasmas transfected cells or natural cells are ideal sample for immunoprecipitation.
As same as the enzyme linked immunosorbent assay,the Chemiluminescence assay use capture antibody to absorb antigen and the detect antibody conjuageted HRP react with limino emit light for detect. If the capture antibody is also an IP antibody then conjugated the antibody to a magnetic beads for capturing antigen and condense the target, it will easily be detected by Chemiluminescence assay. With the helping of immunoprecipitation preocedure antigen has been collected, for some cell factors,IL moleculars, this method is a good choice.