Confirmed by Flow Cytometry (FACS)

Sino Biological Inc. hase commit to developing a variety of antibodies, including Immunohistochemistry / IHC antibody, Flow Cytometry / FACS antibody, Immunofluorescence / IF / ICC antibody, Immunoprecipitation / IP antibody, Western Blot / WB antibody and ELISA antibody.

There are a variety of detection methods for antibody, and the method can verify each other. The Immunofluorescence / IF / ICC antibodies can be verified by the method of IHC, FAC(FC) and WB. If one result of IHC,FAC(FC) and WB was correct, Immunofluorescence / IF / ICC antibodies were validated that the the Immunofluorescence / IF / ICC antibody is specific to target.

IF Antibodies Confirmed by Flow Cytometry / FACS

CD146 Catalog: 10115-R044-F

Immunofluorescence staining of Human CD146 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD146 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 4℃ overnight, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Profile of anti-CD146 reactivity on HeLa cells analyzed by flow cytometry.

CD147 Catalog: 10186-MM08-F

Immunofluorescence staining of human CD147 in HeLa cells with mouse monoclonal antibody (15 µg/mL). The image showing membrane staining of HeLa cells. Primary antibody (FITC conjugated) was incubated for 1 hour at 37℃.
Profile of peripheral blood lymphocytes analyzed by flow cytometry. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.

BMP4 Catalog: 10609-MM02

Immunofluorescence staining of Human BMP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP4 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human BMP4 on HeLa cells. Cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP4, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

OLFM4 Catalog: 11639-MM12

Immunofluorescence staining of Human OLFM4 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human OLFM4 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human OLFM4 expression in DU145 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-OLFM4 (11639-MM12, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.