Confirmed by Western Blot / WB

Sino Biological Inc. hase commit to developing a variety of antibodies, including Immunohistochemistry / IHC antibody, Flow Cytometry / FACS antibody, Immunofluorescence / IF / ICC antibody, Immunoprecipitation / IP antibody, Western Blot / WB antibody and ELISA antibody.

There are a variety of detection methods for antibody, and the method can verify each other. The Immunofluorescence / IF / ICC antibodies can be verified by the method of IHC, FAC(FC) and WB. If one result of IHC,FAC(FC) and WB was correct, Immunofluorescence / IF / ICC antibodies were validated that the the Immunofluorescence / IF / ICC antibody is specific to target.

IF Antibodies Confirmed by Western Blot / WB

Beta-Tubulin Catalog: 100109-MM05


Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.

POU2F1 Catalog: 100726-T34


Immunofluorescence staining of POU2F1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human POU2F1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.

TRIM63 Catalog: 100739-T34


Immunofluorescence staining of TRIM63 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TRIM63 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.

MKI67 Catalog: 13180-MM01


Immunofluorescence staining of TRIM63 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TRIM63 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cytoplasm and nucleus.