Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate.
Compared to other immunoassay methods, there are many advantages of ELISA. ELISA tests are more accurate. They are considered highly sensitive, specific and compare favorably with other methods used to detect substances in the body, such as radioimmune assay (RIA) tests. ELISA possesses the added advantages of not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus).
Specific storage recommendations are listed on the Certificate of Analysis. We recommend that all antibodies be aliquoted into smaller aliquotes and stored at -20° C or below. Avoid multiple freeze-thaw cycles as this will affect antibody activity. We cannot guarantee how the performance of the antibodies if they are not stored as stated on the datasheet.
Unless otherwise indicated in the data sheet, most of our antibodies can be stored at 2℃-8℃ for one month without detectable loss of activity and are stable for twelve months from date of receipt when stored at -20℃ to-70℃. Repeated freezing and thawing should be avoided
All Sino Biological Inc.'s ELISA antibodies are supplied as liquid. It remains stable during the delivery process at ambient temperature. We have tested every antibody's stability and activity following a period of more than 7 days at -20°C, room temperature, or 37°C. Antibodies under all three conditions turned out to be equally active and stable.