ELISA Kit Term: Limit of Detection

The ELISA method is a benchmark for quantitation of pathological antigens and there are indeed many variations to this method. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, prematched capture and detector antibodies. The resulting signal provides data which is very sensitive and highly specific.

Limit of Detection

The limit of detection is (LOD) to determine the sensitivity of the kit. Lower LOD means higher sensitivity.The limit of detection is crucial for the use of ELISA Kit.