ELISA Kit Troubleshooting: No Signal

Though without that complicated protocols as IHC, immunoprecipitation etc, ELISA assay might generate many problems as what could occur in most of the immuno assays. This part provides troubleshooting of ELISA kit with means of cause-solution table with problems listed beforehand. Please note that the conditions described here may not pertain to every ELISA kit because performance requirements vary for individual assays. Be sure to check your package insert for specifications.

Possible Sources Solutions
Incorrect or no detection antibody was added Add appropriate detection antibody and continue
Substrate solution was not added Add substrate solution and continue
Incorrect storage condition Check if the kit is stored at recommended condition and used before expiration date