ELISA Kit Troubleshooting: Poor Detection Value

Though without that complicated protocols as IHC, immunoprecipitation etc, ELISA assay might generate many problems as what could occur in most of the immuno assays. This part provides troubleshooting of ELISA kit with means of cause-solution table with problems listed beforehand. Please note that the conditions described here may not pertain to every ELISA kit because performance requirements vary for individual assays. Be sure to check your package insert for specifications.

This section is intended to recommend possible causes and relative troubleshooting for the problem of ELISA poor detection value.

Possible Sources Solutions
The concentration of antigen in samples was too low Enriching samples to increase the concentration of antigen
Samples were ineffective Check if the samples are stored at cold environment. Detect samples in timely manner