Sinobiological Inc. ELISA Kits are complete and ready-to-use. Immunoassays are available for measuring a range of molecules including virus, cytokines, interleukin, chemokines, growth factors, CD molecule and more. The features of the ELISA Kit are easy to use, high sensitivity, high repeatability.
The high sensitivity of ELISA, comes from the enzyme as a reporting group. As is known to all, the enzyme is an organic catalyst, a small amount of which could induce a large span of catalytic reactions to produce observable chromogenic reaction phenomenon. Therefore, this system is often taken as the amplification system of enzyme. By ELISA, a tracer of the antigen or antibody is achieved in the cell or subcellular level, also, antigen or antibody quantification can be done in the microgram or even nanogram levels.
Specificity of ELISA is because of the selectivity of the antibody or antigen. Actually, the binding of antigen or antibody only occurs in the epitope of an antigen or antigen-binding site of an antibody. Since, there is a complementary relationship between epitope and antigen-binding site both in chemical structure and spatial configuration, the reaction between antigen and antibody shows a strong specifity.
Linearity range is used for quantitative calculation of sample value. Through measuring a series of absorbance for standard material that is known concentration, and we can get the curve, and then we can calculate the actual testing sample value .Linearity range is crucial for the use of ELISA Kit.