Enterovirus 71 VP0 Proteins Research Reagents

EV-A71 is one of the major etiological agents causing hand, foot, and mouth disease (HFMD), which generally affects children aged five and below. The icosahedral capsid of EV-A71 consists of 60 identical protomers, each containing four different structural proteins (VP1–VP4). This viral capsid encloses single-stranded positive-sense RNA. During assembly, the P1 polyprotein is cleaved to VP0 (36 kDa), VP1 (32 kDa), and VP3 (27 kDa), and VP0 is subsequently cleaved into VP2 (28 kDa) and VP4 (8 kDa). VP1–VP3 are exposed on the capsid surface and span the thickness of the capsid, while VP4 is located inside the capsid.

Enterovirus 71 VP0 Protein (1)

    Enterovirus 71 VP0 Lysate (1)

      Enterovirus 71 VP0 分子背景

      Human enterovirus 71 genome polyprotein is a member of the picornaviruses polyprotein family. It contains two peptidase C3 domains, one RdRp catalytic domain, one SF3 helicase domain. Genome polyprotein is cleaved into the following 12 chains: Protein VP (VP4-VP2), Protein VP4 (P1A), Protein VP2 (P1B), Protein VP3 (P1C), Protein VP1 (P1D), Picornain 2A (P2A), Protein 2B (P2B), Protein 2C (P2C), Protein 3A (P3A), Protein 3B (P3B), Picornain 3C (Protease 3C) and RNA-directed RNA polymerase 3D-POL (P3D-POL). VP precursor is a component of immature procapsids. Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. All the three latter proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid composed of 6 copies of each VP1, VP2, and VP3, with a diameter of approximately 3 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes.

      Enterovirus 71 VP0 参考文献

      • Brown B.A., et al., 1995, Virus Res. 39:195-206.
      • Tang W.-F. et al., 2007, J. Biol. Chem. 282:5888-5898.
      • Huang,S.C. et al., 2008, Virus Res. 131 (2):250-9.

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