Flow Cytometry (FACS) Antibody CD Molecule


Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection andprotein engineering.
The cluster of differentiation (or cluster of designation), often abbreviated as CD, is a protocol used for the identification and investigation of cell surface molecules present on white blood cells. The cluster of differentiation nomenclature was proposed for the classification of the many monoclonal antibodies generated by different laboratories around the world against epitopes on the surface molecules of leukocytes. Since then, the use of cluster of differentiation has expanded to many other cell types.