Flow Cytometry (FACS) Antibody Cytokine


Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection andprotein engineering.
Cytokines are a large group of proteins, peptides or glycoproteins that are secreted by specific cells of immune system. Cytokine is a general name; other names are defined based on their presumed function, cell of secretion, or target of action. And the cytokine superfamily includes interleukins, chemokines, colony-stimulating factors (CSF), interferons, and the transforming growth factors (TNF) and tumor necrosis factor (TGF) familes.