Flow Cytometry (FACS) APC Conjugated Antibody

Tips for choosing a fluorescently labeled antibodies for multi-color analysis

• Choose the brightest set of fluorochromes for your instrument.
• Minimize the spectral overlap through choosing the right fluorescent labels.
• Combine the brightest label with the protein which has the lowest level of expression, and vice versa.

Fluorochrome Excitation Peak (nm) Emission Peak (nm) Laser Wavelengths (nm)
Allophycocyanin (APC) 650 660 633, 635, 647