The use of flow cytometry in research has boomed since the mid-1980s. Until now, it has been widely used in varies of fields, such as immunology, cell biology, pathology, microbiology, and so on. It can be used for Immunophenotyping, DNA cell cycle/ tumor ploidy, Membrane potential, Ion flux, Cell viability, Intracellular protein staining, pH changes, Cell tracking and proliferation, Sorting, Redox state, Chromatin structure, Total protein, Lipids, Surface charge, Membrane fusion/ run-over, Enzyme activity, Oxidative metabolism, Sulfhydryl groups/ glutathione, DNA synthesis, DNA degradation, Gene expression, et al.. Flow cytometry can be used to detect a variety of different fluorescently labeled cell index, which antigen-antibody reaction based detection method is the most widely used and most sophisticated flow cytometry method.
Flow Cytometry can be used to detect two main aspects of cell as follows:
Content of DNA, RNA and proteins
Hormone Binding Sites
|Cells per field/ sec||Approx 100||20,000|
|No. of parameter||< 6||<24|
|Quantifiable||Maybe (using complex analysis tool) 12-16 bit (< 65,536 channels)||Yes 18 bit resolution (262,144 channels)|
|Ave number of analysed cells||1,000 (10 fields of 100)||> 10,000|