What is Good Result for Cytoplasm Proteins in Immunocytochemistry (ICC)?

The researchers always have the question that what is actually a good result or bad result for immunofluorescence? This question has no single answer because the judgment of result is an unascertainable step. There are many elements to be considered. If molecules or proteins which express only in cell cytoplasm, the immune fluorescence result is can be confirmed exactly. The positive control should be not include nuclear and membranes. However, if the target molecules or proteins express in cell cytoplasm and also in other location, the result is difficult to confirm. But, we know the good immune fluorescence result should have no background. Now, we can see the one example for good and bad immune fluorescence.

Good Result

Target:Human AFP
Bad Results

Target:Human AFP