Examples of Good Western Blot Results

A good Western Blot result is required if researchers want to avoid false positives or negatives. But what are the characteristics of a good Western Blot result? Normally, it means clear and straight bands showed on a membrane. If you have problems in your Western Blotting, please visit "Western Blot Troubleshooting Guide" for advice.

To perform Western Blot successfully, the quality of antibodies are one of the most important factors. Our Western Blot antibodies were extensively validated by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells). Some of these Western Blot / WB antibodies also be confirmed with animal tissues, including mouse, rat and ferret, etc. Sino Biological's Western Blot antibodies have the advantage of high sensitivity, high specificity and high stability.

The following examples are a variety of good Western Blot results which detect target protein in different cell lines, such as A431, HEK293 and MCF-7.

Good Western Blot results from A431 cell line

What is A431 cell line? A431 cell is human epithelial carcinoma cell line which belongs to cancer cells. A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85- year-old female patient. A431 cells have been used for a variety of studies in cell biology and in biomedical research. A431 cell line is often used in Western Blot / WB test.

  • Western Blot result of CCR2 expression in A431 whole cell lysate using anti-CCR2 rabbit polyclonal antibody (Cat: 100450-T32).

  • Western Blot result of KRT14 expression in A431 whole cell lysate using anti-KRT14 mouse monoclonal antibody (Cat: 100129-MM02T).

  • Western Blot result of p53 expression in A431 whole cell lysate using anti-p53 rabbit monoclonal antibody (Cat: 90001-R014).

  • Western Blot result of ACTA2 expression in A431 whole cell lysate using anti-ACTA2 rabbit polyclonal antibody (Cat: 100125-RP02).

Good Western Blot results from HEK 293 cell line

Where do HEK cells come from? HEK 293 is a cell line developed from human embryonic kidney cells, also known as HEK cells. HEK 293 cells are used widely in biology research because HEK 293 cells are very easy to been cultured and transfected. Almost every one of who have cultured cells knows HEK 293 cells. HEK 293 cell line is often used in Western Blot / WB test.

  • Western Blot result of CD207 expression in 293T whole cell lysate using anti-CD207 rabbit polyclonal antibody (Cat: 100414-T32).

  • Western Blot result of PDGFRA expression in 293T whole cell lysate using anti-PDGFRa mouse monoclonal antibody (Cat: 10556-MM14).

  • Western Blot result of NANOS1 expression in 293T whole cell lysate using anti-NANOS1 rabbit polyclonal antibody (Cat: 100529-T32).

  • Western Blot result of ACBD6 expression in 293T whole cell lysate using anti-ACBD6 mouse monoclonal antibody (Cat: 11112-MM09).

Good Western Blot results from MCF-7 cell line

What are MCF-7 cells? MCF-7 cells are human breast adenocarcinoma cell line belongs to cancer cells. MCF-7 was developed in 1970 from a 69-year-old Caucasian woman. MCF-7 is Michigan Cancer Foundation-7. MCF-7 can live for longer than a few months. This feature is helpful for the cancer researchers. MCF-7 cell line is often used in Western Blot / WB test.

  • Western Blot result of CD240CE expression in MCF-7 whole cell lysate using Anti-CD240CE rabbit polyclonal antibody (Cat: 101328-T32).

  • Western Blot result of CD196 expression in MCF-7 whole cell lysate using anti-CD196 rabbit polyclonal antibody (Cat: 100452-T32).
     

  • Western Blot result of LXN expression in MCF-7 whole cell lysate using anti-LXN mouse monoclonal antibody (Cat:10211-MM04).

  • Western Blot result of Galectin 3 expression in MCF-7 whole cell lysate using anti-Galectin 3 rabbit polyclonal antibody (Cat: 10289-RP02).

Good Western Blot results from Hela cell line

Hela cell line is the human cervical carcinoma cell line.Hela cell line is the oldest and most commonly used human cancer cell line which is successfully cloned in 1955. Hela cell line is widely used in biology research and biomedical research field which was found contaminate many other cell lines in laboratory. Hela cell line is often used in Western Blot / WB test.

  • Western Blot result of CD240CE expression in Hela whole cell lysate using anti-CD240CE rabbit polyclonal antibody (Cat: 101328-T32).

  • Western Blot result of IL17RA expression in Hela whole cell lysate using anti-IL17RA mouse monoclonal antibody (Cat: 10895-MM07).

  • Western Blot result of SMARCC1 expression in Hela whole cell lysate using anti-SMARCC1 rabbit polyclonal antibody (Cat: 100848-T36).

  • Western Blot result of GATAD2A expression in Hela whole cell lysate using anti-GATAD2A rabbit polyclonal antibody (Cat: 100924-T32).

Good Western Blot results from A549 cell line

A549 is a human adenocarcinomic human alveolar basal epithelial cell belongs to cancer cells. A549 cell line was first established in 1972. The developers D. J. Giard, et al.) removed and cultured lung tissue in the explanted tumor of a 58-year-old caucasian male. A549 have a modal chromosome number of 66.A549 cell line is often used in Western Blot / WB test.

  • Western Blot result of AARSD1 expression in A549 whole cell lysate using anti-AARSD1 rabbit monoclonal antibody (Cat: 14280-R012).

  • Western Blot result of LXN expression in A549 whole cell lysate using anti-LXN rabbit monoclonal antibody (Cat: 10211-R101).

  • Western Blot result of LTA4H expression in A549 whole cell lysate using anti-LTA4H rabbit monoclonal antibody (Cat: 10276-R201).

  • Western Blot result of KYNU expression in A549 whole cell lysate using anti-KYNU rabbit polyclonal antibody (Cat: 10841-T52).

Good Western Blot results from Jurkat cell line

What are Jurkat cells and why are they used? Jurkat cells ia a human peripheral blood leukemia T cells. Jurkat cells is a suspended cell. Jurkat cell line is developed in 1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. Jurkat cells are often used in science study because of their ability to produce interleukin 2. Jurkat cells are used in biological research to confirm the mechanism of differential susceptibility of cancers to drugs. Jurkat cell line is often used in Western Blot / WB test.

  • Western Blot result of ICAM1 expression in Jurkat whole cell lysate using anti-ICAM1 rabbit polyclonal antibody (Cat: 10346-RP02).

  • Western Blot result of GLUL expression in Jurkat whole cell lysate using anti-GLUL rabbit polyclonal antibody (Cat: 100715-T44).

  • Western Blot result of LCK expression in Jurkat whole cell lysate using anti-LCK rabbit polyclonal antibody (Cat: 100817-T32).

  • Western Blot result of IL2RG expression in Jurkat whole cell lysate using anti-IL2RG rabbit polyclonal antibody (Cat: 105167-T40).

Good Western Blot results from HepG2 cell line

What is an HepG2 cell line? HepG2 cell line is a human liver carcinoma cell line derived from liver cancer tissue of a 15 year old Caucasian. HepG2 secrete a variety of plasma proteins: albumin, alpha 2-macroglobulin, plasminogen, transferrin etc. HepG2 cells have been always used in biological study as in vitro model system for the research of liver related diseases. HepG2 cell line is often used in Western Blot / WB test.

  • Western Blot result of Chromogranin A expression in HepG2 whole cell lysate using anti-Chromogranin A mouse monoclonal antibody (Cat: 100256-MM03).

  • Western Blot result of beta-III Tubulin expression in HepG2 whole cell lysate using anti beta-III Tubulin rabbit polyclonal antibody (Cat: 101288-T36).

  • Western Blot result of HSP27 expression in HepG2 whole cell lysate using anti-HSP27 mouse monoclonal antibody (Cat: 10351-MM01).

  • Western Blot result of ENO3 expression in HepG2 whole cell lysate (Cat: 14270-MM02) using anti-ENO3 mouse monoclonal antibody.

Good Western Blot results from hESC cell line

Human Embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) research require careful attention to culture conditions. High activity cytokines and growth factors are important for culture and differentiation of ESCs and iPSCs(e.g., bFGF and TGFB1 with high purity, high activity and low endotoxin.) Embryonic stem cells (ESCs) are stem cells derived from the undifferentiated inner mass cells of the blastocyst which is an earyly-stage embryo. ESCs are capable of unlimited self-renewal by symmetric division. ESCs are pluripotent stem cells that have the ability to proliferate indefinitely in vitro in the undifferentiated in vitro and in vivo to cell types representative of all three primary germ layers: mesoderm, endoderm, and ectoderm. In other words, ESCs can develop into each of the more than 200 cell types of the adult body. hESCs cell line is often used in Western Blot / WB test.

  • Western Blot result of Sox2 expression in HESS9 whole cell lysate using anti-Sox2 rabbit polyclonal antibody (Cat: 100106-RP02) at 1:500 dilution and goat anti-rabbit IgG H&L (Dylight800) at 1/10000 dilution.

  • Western Blot result of NANOG expression in HESS9 whole cell lysate using Anti-NANOG rabbit polyclonal antibody (Cat: 101286-T34) at 1:500 dilution and goat anti-rabbit IgG H&L (Dylight800) at 1/10000 dilution.