Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).
Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.
The A549 cell line was first developed in 1972 by D.J. Giard, et al., through the removal and culturing of cancerous lung tissue in the explanted tumor of 58-year-old Caucasian male. A549 cells have been well characterized over the years and are a valuable tool to researchers that are used as models for the study of lung cancer and the development of drug therapies against it. A549 cell line is often used in Immunofluorescence / IF / ICC test.
|FASN in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FASN polyclonal antibody (1 µg/mL) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.|
|Human CD155 in A549 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD155 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 4℃ overnight, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.|