Immunofluorescence (IF/ICC) Antibody Features: Antigen blocking assay

Sino Biological Inc. is committed to developing a variety of antibodies, including Immunohistochemistry / IHC antibody, Flow Cytometry / FACS antibody, Immunofluorescence / IF / ICC antibody, Immunoprecipitation / IP antibody, Western Blot / WB antibody and ELISA antibody. Each application of the antibodies is a rigorous screening and quality control testing. Sino Biological Inc. has developed 1700 high quality Immunofluorescence / IF / ICC Antibodies which have been test strictly. The test images of the Immunofluorescence / IF / ICC Antibodies are shown on website for the user's reference.

Immunofluorescence / IF / ICC antibodies of Sino Biological Inc. have six main characteristics. The first one is that the antibodies have better affinity and sensitivity than Immunofluorescence / IF / ICC antibodies of other leading brands. Immunofluorescence / IF / ICC antibodies of Sino Biological Inc. have been validated by multiple cell types when the antibodies been test. Some Immunofluorescence / IF / ICC antibodies have been further verification by strict negative controls. Sino Biological Inc. has good cofocal platform for detection of Immunofluorescence / IF / ICC antibodies that ensure the pictures and images are fine and clear. Sino Biological Inc. has other test platform for antibody test which can confirm Immunofluorescence / IF / ICC antibodies based on other mechanism. Immunofluorescence / IF / ICC antibodies have been confirm by antigen blocking assay base on Sino Biological Inc. 's large antigen library.

The specificity of the antibody is very important, and it is more advantageous to get better results. One of the most common and effective methods to test the specificity of antibodies is the antigen blocking test. In the process of antibody and natural antigen binding, the combination of blocking antigen and antibody is used to block the binding of antibodies and specific antigen. If the binding of antibodies and natural antigens can be blocked by the characteristics of the antigen, the binding of antibodies and antigens is specific, and the specificity is good. Antigen blocking test is an effective method for all the antibodies that are used, including the specificity of the Immunofluorescence / IF / ICC antibody. For Immunofluorescence / IF / ICC antibody, antigen blocking experiments refer to incubation of antigen and antibody and then the mixture were added to the natural cells or tissues make antibodies and natural antigen binding. The blocking antigen include protein or polypeptide which have been used to prepared the Immunofluorescence / IF / ICC antibody.

Antigen blocking test is a very effective method to prove the specificity of the antibodies. If the binding of antibodies to the natural antigens can be blocked by the addition of free antigens, it can be shown that the antibody is specific for the specific recognition of the antigen, not nonspecific binding or binding to other antigens. Immunofluorescence / IF / ICC antibody can been confirmed again with antigen blocking. The correct of immunofluorescence staining disappeared after antigen blocking, that mean the Immunofluorescence / IF / ICC antibody has better specificity

Antigen blocking assay

Cytplasm

Nucleus