Immunofluorescence (IF/ICC) Antibody-CHO

Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).

Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.

CHO is abbreviation of Chinese hamster ovary which is is used widely in the biological engineering cells. CHO cells have been established from the ovary of the Chinese hamster and have been used in medical or biological research. CHO cells have been cultured monolayer. Now,CHO cells are used most commonly for industrial production of recombinant protein therapeutics. CHO cell line is often used in Immunofluorescence / IF / ICC test.

Catalog: 13105-MM05

Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).

Catalog: 13105-MM01

Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).

Catalog: 11836-R213

Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.