Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells).
Immunofluorescence / IF / ICC is a laboratory technique that uses antibodies to detect specific peptides or protein antigens in the cell via specific epitopes. Immunofluorescence / IF / ICC allows researchers to evaluate whether or not cells express the target antigen. Moreover, Immunofluorescence / IF / ICC allow researchers to know sub-cellular location of target antigen.
Human Embryonic stem cells (HESCs) and induced pluripotent stem cells (iPSCs) research require careful attention to culture conditions. High activity cytokines and growth factors are important for culture and differentiation of ESCs and iPSCs(e.g., bFGF and TGFB1 with high purity, high activity and low endotoxin.) Embryonic stem cells (ESCs) are stem cells derived from the undifferentiated inner mass cells of the blastocyst which is an earyly-stage embryo . ESCs are capable of unlimited self-renewal by symmetric division. ESCs are pluripotent stem cells that have the ability to proliferate indefinitely in vitro in the undifferentiated in vitro and in vivo to cell types representative of all three primary germ layers: mesoderm, endoderm, and ectoderm. In other words, ESCs can develop into each of the more than 200 cell types of the adult body. HESCs cell line is often used in Immunofluorescence / IF / ICC test.
|OCT4 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OCT4 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.|
|nanog in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human nanog polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.|