Sino Biological Inc. have advanced antibody development platform, including Immunohistochemistry / IHC, Flow Cytometry / FACS, Immunofluorescence / IF, Immunofluorescence / IF/ ICC, Immunoprecipitation / IP, Western Blot / WB and ELISA. Sino Biological Inc.'s Immunofluorescence / IF / ICC antibody development platform have two main types of immunofluorescence imaging. Sino Biological Inc. has developed nearly 1700 Immunofluorescence / IF / ICC antibodies, this provides an effective tool for scientists. These antibodies include different research areas, including cancer, stem cells, diagnostic and Biomarkers etc.. The antibodies were extensive validation by multiple cell types, including cancer cells, such as Hela, MCF7 and HepG2 etc., and stem cells, such as HESC (human embryonic stem cell) and MSC (mesenchymal stem cells)
Different proteins were expressed in different cells. The expression of protein in the cell mainly includes the cell membrane, cytoplasm and nucleus. But some proteins are also expressed on the cell organelles, such as the Golgi body, lysosome, and so on.
In addition to the preparation of a number of proteins on the cell membrane, cytoplasm and nucleus, we have prepared antibodies that can identify the proteins expressed in the cell organelle, including the mitochondrion. The results show that our antibodies can be used for the quality control of target proteins on the mitochondrion.
Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human Diablo monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.