Immunofluorescence (IF/ICC) Tips: Cell Multiple staining

The expression of target protein in the cell can be detected and located by using Immunofluorescence / IF/ ICC. Specificity is very important for Immunofluorescence / IF / ICC antibodies. The results of Immunofluorescence / IF / ICC experiment was determined by the specificity of the Immunofluorescence / IF / ICC antibody. There are many methods to verify the specificity of immune fluorescent antibody, one of which is the method of multiple cell verification which mean the antibodies would be extensive validate by multiple cell types. The expression of target proteins in different cells may be different, some of which are high, some of which are low. Multiple cell verification refers to the use of a fluorescent antibody to detect multiple different cells on the target proteins to confirm this antibody can get the correct verification results in all cells. Of course, not all of the Immunofluorescence / IF / ICC antibodies need to carry out multiple cell verification, some of the location of the target protein expression and in what cells are very clear, so it does not need to carry out the verification of multiple cells. Otherwise, some target proteins expressed by the cell types and the location of the expressed in different cells are not be determined, the multi cell verification will be necessary. However, because of the limitation of cell type or the restriction of the culture of the cell, the antibody of the Immunofluorescence / IF / ICC in many markets did not use the natural cells to verify or use only one kind of cell. Some of Sino Biological Inc. immune fluorescent antibodies have been extens
Sino Biological's Immunofluorescence / IF / ICC antibodies were validated with nature cells. The cells used in Immunofluorescence / IF / ICC include multiple tumor cell lines, such as Hela,MCF7, HepG2 and A431,and so on, and include multiple stem cells, such as human embryonic stem cell (HESC) and human mesenchymal stem cell (MSC).