IHC Antibody Features: Consistency between Different Batches

Now, biologists are aware of the problem of repetitive in the basic research. Perhaps part of the responsibility lies with the antibody, which also contributed to the validation of the antibody. Although the reproducibility of biomedical research is not too high, but if it less than 50%, you would not believe. However, according an article, published in "PLOS Biology" on July, is indeed the case. According to Amgen team, nearly 90% results of published, such as pathways and oncogenes, cannot be repeated by the Amgen scientists. This is not only a waste of research funding, but also greatly delay the scientific discoveries.

The properties of the antibody must be consistent among different batches, it is another important element of high quality antibodies. Different batches of Sino Biological antibodies must undergo strict quality control before they can be sold, to ensure that customers can get correct results with any batch of the antibodies.

Due to different about immunization of animals and their rearing environment, various batches of polyclonal antibodies are different in immunohistochemical staining. Although monoclonal antibodies are secreted by the same B lymphocytes, but the chang of cell culture conditions and antibody purification method will resulting in a change in properties of the antibody and therefore whether it is polyclonal antibodies or monoclonal antibody stability of antibodies between batches is very important.

Examples of consistency between different batches

Batch 1 Batch 2 Batch 3
 

Tissue: brain      Target: Olig-2

Batch 1 Batch 2 Batch 3