IHC Antibody Features-Suitable for Additional Applications

For manufacturers, the preparation of the antibody is not rate-limiting step. The main limitation is to prove that the antibody can identify the intended target, and detect their potency. To prepare qualified agents, there must be high-throughput validation after high-throughput antibody production.
There are a variety of detection methods for antibody, and the method can verify each other. The immunohistochemistry / IHC antibodies can be verified by the method of IF, FAC(FC) and WB. If one result of IF,FAC(FC) and WB was correct, immunohistochemistry / IHC antibodies were validated that the immunohistochemistry / IHC antibody is specific to target.

Examples of multiple application

LAMP1 / CD107a Antibody: suitable for IHC, FACS, WB and IF      catalog:11215-R107

Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.

EpCAM / TROP-1 / TACSTD1 Antibody: suitable for IHC, FACS, WB and IF      catalog:10694-R028

Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LAMP1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.
Flow cytometric analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunochemical staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).