IHC Antibody Features: High Specificity

Tissues used in immunohistochemistry is more complex, each tissue slices often contain dozens or hundreds of antigen, then how to prove antibodies binding to target protein specifically but not binding to other proteins in the tissue? We verify this by antigen blocking.
Antigen blocking test is a very effective method to prove the specificity of the antibody. If the binding of antibodies to the natural antigens can be blocked by the addition of free antigens, it can be shown that the antibody is specific for the specific recognition of the antigen, not nonspecific binding or binding to other antigens.

Non-antigen blocking Antigen blocking

Examples of antigen-blocking

AGT: 10994-RP02

Immunochemical staining of human AGT in human cirrhosis with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).The left panel: tissue incubated with primary antibody;The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

CD21: 10811-R005

Immunochemical staining of human CD21 in human tonsil with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to germinal center. The left panel: tissue incubated with primary antibody;The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

GOLM1: 13066-RP02

Immunochemical staining of human GOLM1 in human liver with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).