Immunohistochemistry (IHC) Antibody-Heart Tissue

Immunohistochemistry, which the basic principles is immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.

The heart is the most important organ in humans and other animals; the main function is to provide pressure, which pumps blood through the blood vessels to various parts of the body in order to supply oxygen and nutrients and take away metabolic end products (such as carbon dioxide, an inorganic salt, urea, and uric acid, etc.), so that cells maintain normal metabolism and function. In humans, mammals and birds heart is divided into four chambers: left, right atrium; and lower left and right ventricles. As such a complex organ heart is prone to some cardiovascular disease some become increasingly common with aging. Obesity, hypertension and high cholesterol can increase the risk of heart disease, Coronary artery disease is also known as ischemic heart disease or atherosclerosis, it is the most common form of heart disease. Cardiomyopathy and most commonly dilated cardiomyopathy refers to the heart muscle contractility deterioration. It is a common cause of heart failure. Myocarditis is inflammation of the heart; this may be specific to the region as a pericarditis, myocarditis, and endocarditis, or it can be of the whole heart known as pancarditis. A variety of target molecules expressed on cardiac tissue is used for the clinical diagnosis of heart disease, heart tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect heart disease.

Different species of heart slices as follows: