Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
Lymph nodes is unique organs to mammals. Lymph node is an oval organ lymphatic system, widely distributed throughout the body, There are hundreds of lymph nodes in the human body. They are located deep inside the body, such as around the lungs and heart, or closer to the surface, such as under the arm or groin. Lymph nodes are major sites of B, T, and other immune cells. The primary function of all lymph nodes is the production of lymphocytes, which help defend the body against microorganisms and against harmful foreign particles and debris from lymph before it is returned to the blood stream. Lymph nodes do not deal with toxicity, which is primarily dealt with by the liver and kidneys. Lymph nodes also have clinical significance. They inflamed or enlarged in various infectious diseases which rang from the trivial throat infections to life-threatening cancer. The condition of the lymph nodes is very important in cancer staging, which decides the treatment to be used, and determines the prognosis. When swollen, inflamed or enlarged, lymph nodes can be hard, firm or tender.A variety of target molecules expressed on lymphgland tissue is used for the clinical diagnosis of spleen disease, lymphgland tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect lymphgland disease.
Different species of lymphland slices as follows: