Immunohistochemistry, using the basic principles of immunology - the antigen-antibody reaction, namely antigen and antibody specific binding principle, by chemical reaction of the labeled antibody reagent (luciferase, an enzyme, metal ions, isotopes) to confirm the antigens (peptides and proteins) in tissues, its location, qualitative and quantitative research, called immunohistochemistry (immunohistochemistry) or immunocytochemistry techniques (immunocytochemistry).It combined the specific of immune response with visibility of histochemistry by means of a microscope (including fluorescence microscopy, electron microscopy) imaging and amplification, detection of various antigens in a cell, subcellular level, such as proteins, polypeptides, enzymes, hormones, pathogens, and receptors. Immunohistochemical techniques have developed rapidly in recent years. It was limited to immunofluorescence techniques in 1950s, but gradually developed after the 1950s to establish a highly sensitive and more practical immunization enzyme technology.
The spleen is an organ in almost all vertebrates in existence. The spleen is an organ above your stomach and under your ribs on your left side. The spleen is the largest organ in the lymphatic system. Structure of spleen is similar to a large lymph node, spleen's main function is to act as a filter for blood. It controls the amount of red blood cells and blood storage in the body, and helps to fight infection. The spleen is composed of red pulp and white pulp. The white pulp is part of the infection-fighting (immune) system. It produces white blood cells called lymphocytes, which in turn produce antibodies The red pulp is responsible for purifying the blood and removing dead or old blood cells. The red pulp contains other white blood cells called phagocytes that ingest microorganisms, such as bacteria, fungi, and viruses. The spleen can become swollen after an infection or injury, or because of a disease such as cirrhosis, leukaemia or rheumatoid arthritis. You can also damage or rupture your spleen in an injury, especially if it is already swollen. People can live without a spleen. When the spleen is removed, Other organs, such as liver, will take over some of the spleen's work. Without a spleen, however, body will lose some of its ability to fight infections.A variety of target molecules expressed on spleen tissue is used for the clinical diagnosis of spleen disease, spleen tissue is commonly used in immunohistochemical detection. IHC is an important means to be widely used to detect spleen disease.
Different species of spleen slices as follows: