Immunohistochemistry (IHC) Introduction

Immunohistochemistry (IHC) is a method used to determine the expression of biomarkers in tissue in formalin-fixed, paraffin-embedded (FFPE) tissues based on antigen–antibody interaction. Since immunohistochemistry involves specific antigen-antibody reaction, it has apparent advantage over traditionally used special enzyme staining techniques that identify only a limited number of proteins, enzymes and tissue structures. Therefore this technique is widely used in dermatologic diagnostics and research, and its applications continue to be extended because of its ease of use, reliability, and versatility.