Immunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other techniques such as gel filtration or density gradient sedimentation. The source of antigen for immunoprecipitation can be unlabeled cells or tissues, metabolically or intrinsically labeled cells, or in vitro-translated proteins. This unit describes a wide range of immunoprecipitation techniques, using either suspension or adherent cells lysed by various means.Usually, plasmas transfected cells or natural cells are ideal sample for immunoprecipitation.
|Organism Type||Human||These cells are isolated from human embryonic kidneys (HEK). The 293T cells are transformed with large T antigen.|
|Application||Cell Culture / Growth Conditions,Protein Expression,RNAi,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection|