Protein Purification Immunoprecipitation (IP)

Immunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other techniques such as gel filtration or density gradient sedimentation. The source of antigen for immunoprecipitation can be unlabeled cells or tissues, metabolically or intrinsically labeled cells, or in vitro-translated proteins. This unit describes a wide range of immunoprecipitation techniques, using either suspension or adherent cells lysed by various means.Usually, plasmas transfected cells or natural cells are ideal sample for immunoprecipitation.

Following immunoprecipitation procedure, we can conjugated our IP antibody to magnetic beads or agaros beads, if we collect the beads we also collect the antibody and antigen after the antibody binding to antigen, then we elute the target protein from antibody, the antigen was purified .