Western Blotting (protein blotting or immunoblotting), is an important technique to detect specific proteins in a sample of tissue homogenate or extract. When performing a Western Blot, it's a wise idea to follow your procedure step by step.
To perform a Western Blot successfully, every single step should not be neglected. It includes: (1) WB buffers preparation, (2) samples preparation, (3) gel electrophoresis, (4) protein transfer, (5) membrane blocking, (6) antibody incubation, (7) WB detection and imaging, (8) Data analysis.
If you have any issues in your Western Blotting, please contact firstname.lastname@example.org.