Black Dots Troubleshooting in Western Blots

Western Blot / WB is a widely used analytical technique used in molecular biology and enables researchers identify specific proteins in given samples. However, black dots will appear in a Western Blotting if antibodies bind to the Western Blot blocking solution. Why are there black dots in a Western Blot / WB? The following Western Blot troubleshooting guide is supposed to help solving black dot issues in your western blotting.

Western Blot possible causes & solutions for black dots

Black dots in a western blot

Black dots in a Western Blot
Possible cause Solution
Antibodies bind to the western blot blocking solution • Filter the blocking solution ahead of time.