Western Blot / WB is a widely used analytical technique used in molecular biology and enables researchers identify specific proteins in given samples. However, black dots will appear in a Western Blotting if antibodies bind to the Western Blot blocking solution. Why are there black dots in a Western Blot / WB? The following Western Blot troubleshooting guide is supposed to help solving black dot issues in your western blotting.
Black dots in a Western Blot
|Antibodies bind to the western blot blocking solution||• Filter the blocking solution ahead of time.|