White Bands Troubleshooting in Western Blots

Western Blot / WB is a commonly analytical method to detect and analyse specific proteins. Researchers often encounter various troubles during Western Blot analysis. For example, white bands may appear in a Western Blot. But why are there white bands in a Western Blot / WB? The following Western Blot troubleshooting guide is supposed to give you possible causes and corresponding solutions and assist you get reliable results in your western blotting.

Western Blot possible causes & solutions for white bands

White bands in a western blot

White bands in a Western Blot
Possible cause Solution
Too much antibody (primary antibody or/and secondary antibody) or excess loaded amount of proteins • Reduce the amount of antibody and loaded proteins during western blotting.